Merlene Ann babu

Merlene Ann babu

Assistant Research Scientist
Kuwait

About Me

Assistant Research Scientist at the Kuwait Institute for Scientific Research with a Ph.D. in Biosciences and Technology from VIT University, Vellore Institute of Technology. Experienced in biotechnology, plant physiology…

Experience

Assistant Research Scientist

Kuwait Institute for Scientific Research
Jun 2014 - Present · 12 years 1 month

Assisted the project leader in the preparation of project proposals
Assisted the project leader in the execution of various project tasks
Conducted routine monitoring of field experiments
Conducted routine monitoring of laboratory experiments
Documented results
Performed statistical analysis of data
Prepared reporting for client-funded projects
Worked on improving barley and sorghum production under harsh environmental conditions using mutation breeding techniques
Worked on improving production and fertilizer use efficiency of forage crops with nuclear techniques
Worked on implementing mutation induction to improve barley production under harsh environmental conditions
Worked on improving production and water use efficiency of forage crops with nuclear techniques
Worked on enhancing the use of salt affected soils and saline water for crop and biomass production
Worked on reducing land and water quality degradation in ARASIA states parties
Worked on enhancing wheat and barley productivity through induced mutation with supportive breeding and related biotechnology techniques
Worked on nitrogen use efficiency of mutant barley genotypes in Kuwait
Worked on agronomic performance of mutant barley genotypes in Kuwait
Worked on abiotic effects on mutant barley
Worked on integrated utilization of cereal mutant varieties in crop/livestock production systems

Assistant Researcher

Kuwait Institute for Scientific Research
Apr 2014 - Present · 12 years 3 months

• Proximate Analysis: Determination of protein, fat, moisture, ash and fiber content in food samples
• Plant Physiology & Molecular Biology: Mutation Breeding, Use of nitrogen and carbon stable isotopes for soil and water management, Recombinant DNA Technology, Quantitative Real Time PCR, Gene Cloning, Gene Silencing Via RNA Interference Cryopreservation, RNA and DNA Isolation, Bacterial Transformation Agarose and Polyacrylamide Gel Electrophoresis, Protein Extraction and Quantification, Enzymatic Assays, Enzyme Immobilization
• Microbiology: Preparation of microbiological media and sterilization, Aseptic techniques of inoculation of agar plates and broth, Isolation and culture of microorganisms, Serial dilution method, Plate count method, Using a spectrophotometer and microscope, Gram’s staining, Biochemical tests, Anti-bacterial assays
• Plant Tissue Culture: Surface Sterilization, Media Preparation, In-Vitro Regeneration of Plants. Agrobacterium Mediated Transformation of plants, Extraction of various health beneficial Phytochemicals from various plants, Qualitative and Quantitative determination of Phytochemicals, Determination of anti-oxidant and anti-bacterial effects of plant extracts.

Lecturer in the Biotechnology Department

Vinayaka Mission’s Kirupananda Variyar Engineering College-Salem, Tamilnadu, India
Jun 2023 - Dec 2013 · 9 years 6 months

Research Fellow

VIT University, Vellore Tamil Nadu
Dec 2008 - Apr 2013 · 4 years 4 months

Acquired Ph.D. degree by April 2013 in the School of Bio Science and Technology

Trainee

Piramal Life Sciences Ltd, Mumbai

Skills

Project Works Data Analysis and Reporting Mutation Breeding Stable Isotopes Recombinant DNA Technology Quantitative Real Time PCR Gene Cloning Gene Silencing RNA Interference Cryopreservation RNA Isolation DNA Isolation Bacterial Transformation Agarose Gel Electrophoresis Polyacrylamide Gel Electrophoresis Protein Extraction Protein Quantification Enzymatic Assays Enzyme Immobilization Microbiology Aseptic Techniques Serial Dilution Plate Count Method Spectrophotometry Microscopy Gram Staining Biochemical Tests Antibacterial Assays Plant Tissue Culture Surface Sterilization Media Preparation In-Vitro Regeneration Agrobacterium-Mediated Transformation Phytochemical Analysis Antioxidant Assays HPLC Paper Chromatography Thin Layer Chromatography ELISA
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