Highly qualified molecular biologist specialized in genomics for more than nine years with Team supervising and Lab management experience. Worked on population genetics and NGS assay validation research projects. Experie…
Highly qualified molecular biologist specialized in genomics for more than nine years with Team supervising and Lab management experience. Worked on population genetics and NGS assay validation research projects. Experienced in Next Generation Sequencing (NGS) on the Illumina Miseq, Ion Torrent, MGI, Nanopore sequencing platforms, Sanger Sequencing and DNA profiling. Having analytical and problem-solving skills, and technical and administrative and Lab management skills. Seeking a challenging technical / research /management position that will capitalize my skills, education, and experience.
Chip seq, nanopore seq, illumina seq, ddPCR, q pcr, cell culture,lab admin and management. Short and long read sequencing, 10x Genomics, single cell seq, tapestation, bioanalyzer, DNA/RNA extraction, sample servicing , training students etc
Sample analysis services for researchers and non-academic users
ChIP sequencing by Illumina
Oxford Nanopore (MinION) sequencing
ATAC sequencing by 10X Genomics
Cell Culture of Suspension and Adherent cell lines (K562 & HCT116)
Administrative support and Lab management
ddPCR training and sample analysis for researchers and non-academic users
Working mainly on technical and application support, technical troubleshoots, and product promotions
MGI- NGS sequencing Instruments and library prep kits
MGI Automatic sample Handler for RNA extraction and analysis for Covid RT-PCR testing and Covid qPCR assays
Cytometry Analysis
Worked mainly on technical and application support, technical troubleshoots and product promotions
Thermo Fisher NGS assay applications library prep and technical troubleshoots for Ion Torrent Chef and GeneStudio S5 series platforms
Sanger sequencing assays for single gene mutation/variant analysis using Seqstudio, ABI3500 and ABI3730
TaqMan assays for gene expression and SNP detection using ABI7500 and Quantstudio 5, 7 Flux
STR genotyping training and assay technical troubleshoots for HID and clinical applications
Robotic liquid handling Instruments like ThermoFisher -KingFisher, Pathotrix Auto, Hamilton NGS star and Qiagen & Promega Instruments
GenDX Chimerism qPCR-based assays
All other Thermo and other collaborated company products
Fast Track Diagnostics (FTD) qPCR based clinical assays for pathogen detection
Worked mainly on the assay validation, Tech transfer, and Quality Management of techniques to expand the Bangalore lab activities
MLPA assays for Micro/Major deletion
Sanger sequencing assays for single gene mutation/variant analysis
Fragment analysis assays for mutation detection and confirmation
NGS assay mutation detection and sanger confirmation
Maternal Cell Contamination assays
Validation report writing and SOP preparation as per CAP guidance
Managing and supervising a team for project completion and reporting
Worked on different research projects like the ‘Validation of Cell-Free DNA (cfDNA) Assay for Liquid Biopsy samples’ and ‘Validation of 152 Somatic Cancer Gene Assay’
In charge of the 152 Somatic Gene panel production samples as well
Managing and maintaining the production samples received for 152 Somatic Gene panel assay
Validated the different mutations and gene changes like amplification, deletion and translocation by using qPCR and Sanger sequencing methods
Involved in the standardization of the 152 Somatic Gene panel for detecting the somatic mutations at the level of 5% to below 2% in NGS and copy number and translocation (fusion) changes
Involved in the Inter/Intra operator/kit etc test for validating the assay
Experienced in the various DNA library preparation methods for NGS sequencing
Limit of Detection (LOD) studies of the custom-designed cancer panel on various cell line and Coriell DNA samples for their mutation detection level in the selected gene target sequences, different DNA and library quantization methods
Involved in validating the assay for cfDNA extraction, recovery and mutation detection by ddPCR single gene and multiplex gene assays
ddPCR assay confirmation of the mutation found in the NGS data and RNA extraction and cDNA conversion for qPCR assays
Standardized an alternative method for FFPE Infinium QC Assay and qPCR-based assay for GC rich regions for confirming mutations
Overall, Lab Maintenance and supervising the junior employees and lab Assistants
Worked on the research project named ‘Analysis of Degraded Human DNA by Autosomal Markers with Short Amplicons’
Performed the DNA Extraction, Quantization, Genotyping and Sequencing of STR and SNP markers in the population samples and real samples for population genetic research studies and case reporting
Polymorphic 16 mini/midi STR and 52 SNP loci were selected and analyzed in the collected eleven endogamous population samples of India
Generated the polymorphic autosomal miniSTR & autosomal SNP panels for HID /population Genetic studies
DNA database had been created for the selected Indian populations
Worked on the analysis of the real case samples for DNA reporting
Trained the Scientific Officers on DNA profiling techniques for human identification
Participated in various national conferences and presented papers
Worked as a Lecturer in the Department of Behavioral Health and Allied Sciences to teach B.Sc, M.Sc. and PG Diploma of Forensic Biotechnology students
Taught M.Sc., B.Sc. and PG diploma students for basic biological science subjects and DNA technologies
Took Special Lectures and practical training classes for the Judges and police professionals for DNA profiling
Participated and organized the conferences and workshops conducted in the university
Participated in the curriculum and admission processes
Worked under the Lab Systems, Mumbai to assist in the data analysis and reports
Assisted in the analysis of data collection, analysis and drafting reports
Worked on the Pilot project named ‘Blood mercury concentration in the fisherman populations’
Detected blood mercury level in the fisherman population using UV spectrometry and Mercury Analyzer
Prepared SOPs for various assays
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