Ganiu Idris

Supervised the Medium and Reagent preparationsteamSupervised the Isolation of PBMCs and cryopreservation for immunological studiesSpearhead the cell culture department for all cell-based assaysCarried out Culture of PBMC cell isolatesParticipated in Flow cytometry assaysWriting of Standard Operating Procedures for Immunological and serology assayConducted training on ELISA assayVerification of assay data and transmission to analysis teamReporting laboratory research experimentSupervision of Postgraduates bench work relating to immunology and molecular processServing in company’s Quality Management System team as Equipment Officer

Safety and Immunogenicity of Fractional Doses of Moderna, AstraZeneca &Janssen COVID-19 Vaccine Among Nigerian Adults (SIFCoVAN Clinical Trial Research Project, NIMR) Medium and Reagent preparationsCarried out the Isolation of PBMCs and cryopreservation Carried out the Culture of PBMC cell isolatesParticipated in Flow cytometry assaysConducted Immunological training on ELISA assayVerification of assay data and transmission to analysis teamReporting laboratory research experimentWriting assay reportSample data entry, sorting, and primary analysisMaking orders and requests for lab reagents and consumables.

Nigerian Institute of Medical Research (NIMR) Cell culture lab, Center for Human Virology and GenomicsCoordinated the collection of sewage contaminated water samples (SCW) from different sample sites in Lagos. Conducted the Processing and concentration of collected SCW samples.Frequently carried out the maintenance of mammalian cell culture.Conducted the Inoculation of cultured cells with concentrated SCW samples (suspected virus inoculum).Carried out the microscopic examination of infected cell lines for cytopathic effect (CPE).Carried out Molecular analysis (CDNA, PCR).Carried out gel electrophoresis.Conducted the maintenance of the cell culture laboratory.Making requests from the store.Writing standard operating procedures and reports.

VTM team lead COVID-19 Response team Conducted the preparation, packaging, and delivery of viral transport media (VTM) for NIMR’s COVID-19 sample collection and for commercial use across the nation.Preparation of cell culture media and other reagents.Participated in cytotoxicity assays.Carried out SARS-CoV-2 isolation in mammalian cells.Carried out TCID-50 and plaque assay.Writing an experiment report for Senior research fellows.

Standard operating Procedure development for Laboratory Assays
Carried out PBMC isolation and cryopreservation
Carried out COVID-19 antibody and cytokine Elisa assay
Sorting and logging of received immunological samples data
Primary Data sorting and analysis
Transmission of primary dataset to Secondary data analysis team
Making orders and requests for lab reagents and consumables

Standard operating Procedure development for Laboratory Assays
Specialized in cell culturing and maintenance of cultured cell line for TCID assay, virus culture, plaque assay
Carried out Cytotoxicity assay, drug efficacy test etc.
Coordinated the Viral transport media production team of the Nigeria Institute of Medical Research COVID-19 response team
Serves in capacity of assistant researcher in NIMR Enterovirus (Poliovirus surveillance research)
Coordinated the collection of sewage contaminated water sample
Processing and concentration of viral suspected sample
Nucleic acid Extraction
support in PCR assay
Gel electrophoresis