Employer Active
2 - 4 years
Not Disclosed
Salary Not Disclosed
Saudi Arabian
N/A
1 Vacancy
A fully funded 4-year position is available in the laboratory Structure and Function of Membrane Proteins at the VIB-VUB Center for Structural Biology, Brussels, The project focuses on the structural elucidation of membrane-remodeling protein complexes from the endoplasmic reticulum and integrates fundamental cell biology and structural biology by cryo-electron
The asymmetrical distribution of lipids in biological membranes is a well-established biological paradigm and essential for signaling and organelle-/protein While the regulated asymmetry in the plasma membrane of eukaryotic cells is well-studied, much less is known about the symmetry or asymmetry of lipid bilayers in intracellular The endoplasmic reticulum plays a central role in this context as lipids are retrieved from its cytosolic leaflet for export to acceptor Interleaflet balance and availability of lipid species in the leaflets of the endoplasmic reticulum membrane are governed by scramblases, a functional lipid transporter class (Brunner et , Nature, 2014) that only recently was recognized to be required for non-vesicular lipid transport and as essential host factor(s) for virus
We aim to structurally characterize scramblases involved in lipid export from the endoplasmic reticulum and reveal their roles, organization, and interactome in the In addition, the mechanisms of viral hijacking of scramblase host factors for the assembly and spread of viruses are a matter of
We apply state-of-the-art methods for the expression and purification of delicate membrane protein complexes in cultured mammalian cells and their subsequent biophysical and biochemical characterization by HPLC, SEC-MALS, and functional reconstitution into Proteins will be further characterized in living cells by confocal microscopy located in the For structural work, single particle cryo-electron microscopy is extensively utilized at Belgium s prime location, the Biological Electron Cryogenic Microscopy Center of Flanders (BECM) on the VUB
Candidates must hold a Master s in biochemistry, biophysics, or related A keen interest in eukaryotic cell biology, membrane proteins, and structural biology is essenti The ability to work organized and independently with excellent communication skills is mandatory as well as proficiency in written and spoken Previous experience in structural biology (cryo-EM), membrane protein purification, or cell biology will be Candidates should demonstrate a strong motivation and commitment to solving scientific problems and a curiosity-driven approach in their
Please apply online with a cover letter detailing your background and interest in this position and your full CV including the contact information of two referees (with email, address, and phone number).
For further information visit and contact Janine Brunner ().
Full Time